Coding
Part:BBa_K5374012:Design
Designed by: Langning Xu Group: iGEM24_TestDaily-I-Beijing (2024-09-15)
DB-EGFP (Discoidin Domain). A collagen-binding domain found in extracellular matrix proteins, fused
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 484
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 484
Illegal NheI site found at 924 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 484
Illegal BglII site found at 897
Illegal BamHI site found at 288 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 484
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 484
Illegal NgoMIV site found at 859 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1567
Design Notes
Sequence was optimized for fusion with EGFP, ensuring it retains collagen-binding capacity without disrupting fluorescence for downstream application.
Source
DB domain derived from various ECM proteins, with EGFP from BBa_K1911005.